How To Use Xcyt

This section is still under construction.

Open an image using the "File -> Open Remote" menu option.
Isolate 20-30 cell nuclei. There are different ways to accomplish this.
- Option 1: draw them by hand, using the mouse pointer. Click and drag an approximate outline using the left button. Release the button before reaching the beginning point. Double-click the left button to correct small errors in your boundary. Right-click to remove an outline that did not conform closely to the actual boundary of the necleus.
- Option 2: Choosing the "Edit -> Find Nuclei" option will take several minutes, depending on the speed of your computer, but will do a good job of isolating and outlining the nuclei automatically. Repeated selections of "Find Nuclei" should be very fast. Again, you should delete and re-initialize any the do not perform well.
- Option 3: If you outline a nucleus manually before running "Find Nuclei", the program will search for nuclei of about the same size as the one you outlined. This will result in significantly faster performance, but may miss nuclei of different sizes.
- Option 4: The "Edit -> Speedup" selection runs significantly faster than "Find Nuclei" and covers all possible nuclear sizes but is not as reliable in isolating the nuclei.
Nuclear feature values are displayed automatically on the screen. These can be saved if desired.
To predict whether or not the sample is malignant, choose "File -> Diagnosis." The resulting graph shows a diagnostic index, running from benign cases (on the left) to malignant cases (on the right), and an X to indicate the relative position of the current case.